Liposomes are formulated and processed to differ in size, composition, charge and lamellarity, depending upon method of preparation either active loading technique or passive loading technique. The principle of sonication involves the use of pulsed, high frequency sound waves (sonic energy) to agitate a … methods-of-preparation-liposomes.ppt - Course Hero The MLVs can be broke up into smaller vesicles by the induced pressure.) Analyzing the final product Techniques used for the preparation of liposomes are described below; 1. Preparation of Nano-liposome by Sonication and Pressure Sonication is an easy method of choice in reducing the size of liposomes. Sonication method This method is used in the preparation of SUVs and it involves the subsequent sonication of MLVs prepared by the conventional method either with a bath type or a probe type sonicator under an inert atmosphere, usually nitrogen or argon. Phospholipids can be from natural sources such as soya bean, egg yolk and olive oil. Method This method provided a progress in liposome technology, since it allowed for the first time the preparation of liposomes with a high aqueous space-to-lipid ratio and a capability to entrap a large percentage of the aqueous material presented. Comparison of liposomes formed by sonication and … Methods of liposome preparation General methods of preparation. Preparation of lipid for hydration.
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